Composite

Part:BBa_K3841073:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-10-17)


Expression cassette of pMMO alpha subunit fused to Venus tag with constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
    Illegal PstI site found at 1877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 737
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
    Illegal PstI site found at 1877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1022
    Illegal XbaI site found at 1556
    Illegal PstI site found at 1877
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3841073 is suitable for expression in Komagataella phaffii. For chromosomal integration, insert into a vector backbone of choice and flank BBa_K3841073 by 65bp long homology arms on either site of an induced DSB.

Source

References